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Cotton Case Study: Cotton In Nematode Infested Soil Field Tomatoes Jalapenos Followed by Wheat and Rye: Arizona Ag1000 Case Study
Melons Onions Peanuts
Peppers Pumpkins Case Study: Arizona Soybeans
Sweet Potatoes

Effective Microorganisms® in Field Crops

Combining with Fertilizers
Ag1000 Fertilizer Injection Setup

It is fine to use conventional fertilizers with Ag1000. However, concentrated fertilizers will kill the microbes almost immediately if mixed together in concentrated form. To avoid this, you need to set up separate tanks, one for your Ag1000® and one for the fertilizer. You can use one injection pump to pump from each tank into the irrigation line using a line splitter before the intake of the pump. The dilution with water is great enough to prevent the microbes from dying at that point.
Combining with Pesticides
  • Don't Do It. Pretty much all of your synthetic pesticides on the market kill every one of the microbes in Ag1000®, some do it on contact. With fumigants start applying Ag1000® as close to the end of the residual kill time before applying the Ag1000®.
  • However, with the biological pesticides the two products work well together.
Seed Treatment:
Soak seeds one day before planting in a 1:1,000
Ag1000®/water solution. If the seed cannot be soaked, moisten the seed before planting with the same 1:1,000 Ag1000®/water solution.

Transplanting:
Use as a drench at 4% solution.
Ag1000® can be added with other products, such as organic fertilizers, mineral supplements and compost teas during this process.

Foliar Treatments:
Apply
Ag1000® with a sprayer at a 1.5- 3 gallons per acre diluted with water for coverage. It is best to apply Ag1000® as part of a foliar nutrient program once per week throughout the growing season.

Soil Treatment:
The goal is to get 40 gallons
Ag1000® per acre per year. An easy rule of thumb for growers using irrigation systems is to apply 1-3 gallon of Ag1000® per acre per week, depending on the length of the growing season. Ag1000® can also be applied 5-8 times during the growing season using spraying equipment if irrigation systems are not in use. If continuous cropping, increase the rate to 1 gallon per week for a total of 52 gallons per acre per year.

Post Harvest:
A post-harvest treatment is made at a rate of 1-5 gallons per acre to accelerate lignin and cellulose breakdown of organic matter, which is then turned in prior to next season's sowing.


Cleaning Irrigation Lines:
Clean irrigation lines with an application of 5 gallons per acre, let sit in lines for 4-5 days, and flush. Depending on how hard the water is, this may need to be done 2-4 times per year. The cost of the
Ag1000® is less than sulfuric and it is much safer than sulfuric or bleach.

Well Treatment:
Wells can be treated as well. Inject 55 gallons into a well head and let sit for 3-4 weeks. Flush and repeat one or two more times depending on the color of the water that flushes out. If it clears after the first flush there is no need to repeat the application.



Call TeraGanix for Availability and Pricing of Ag1000®: 866.369.3678.
  • Cotton Case Study: Cotton In Nematode Infested Soil

    Location:

    Mississippi State University Extension Field Mississippi State University Logo

    Contact: H. Randall Smith, Area Agronomist, MCES-MSU

    Enterprises: Cotton

    Treatments:

    10 Gal/Ac applied four times (1 pre-square, 2 square, 1 bloom);

    20 Gal/Ac applied four times (1 pre-square, 2 square, 1 bloom);

    20 Gal/Ac applied six times (1 pre-square, 2 square, 3 bloom);

    60 Gal/Ac at planting;

    Untreated Check

    Agriculture Issues:

    Objectives are to evaluate the effects of Ag 1000 on the fruiting mechanics and yield of cotton grown in reform nematode infested soils without any fertilizer additives.

    Cotton flowering stageCotton PlantOpen Cotton Boll

    This study was done in collaboration with Randy Smith, an agronomist with Mississippi State University. The objectives are to evaluate the effects of Ag1000 on the fruiting mechanics and yield of cotton grown in reform nematode infested soils without any fertilizer additives. This case study is a summarization of Randy Smith’s work and final report from 2013.

    Materials and Methods:

    Planting Date: 6-26-2013

    Cotton variety: PHY 375 WRF

    Harvest Date: 12-5-2013

    Soil type: Commerce Silt Loam

    Rows/plot: two

    Row Spacing: 40”

    Row Length: 40’

    Irrigation: Yes

    Randomized Complete Block Design

    Four Replications

    Treatments:

    10 Gal/Ac applied four times (1 pre-square, 2 square, 1 bloom);

    10 Gal/Ac applied six times (1 pre-square, 2 square, 3 bloom);

    20 Gal/Ac applied four times (1 pre-square, 2 square, 1 bloom);

    20 Gal/Ac applied six times (1 pre-square, 2 square, 3 bloom);

    60 Gal/Ac at planting;

    Untreated Check

    Evaluations Made:

    Pre-square plant map: Plant height (PH), Total nodes (TN), Node of first fruiting branch (NFFB).

    Square plant map:PH, TN, NFFB, % first position retention.

    Bloom Map: PH, TN, NFFB, Nodes above white bloom (NAWB), Node of white bloom (NOWB) and % first position retention.

    Open Boll Map: PH, TN, NFFB, Nodes above cracked boll (NACB), Node of cracked boll (NOCB)

    (ul)Box Mapping which is used to quantify yield and performance by position. Plants from 10 row feet were removed and seed cotton from each position was removed and placed into designated box compartment and a token used to represent each boll for total number by position. Each fruiting position was weighed using gram scales. Both main axis and vegetative bolls are accounted for.

    Results:

    In this study it was shown that 10-20gal/acre applied over 4 applications resulted in the greatest overall plant growth. However, 2 gal/acre whether applied 4x or 6x resulted in the highest overall yield. The application of 60gal/acre showed little benefit verses 10gal/acre and actually showed a decrease in plant growth and yield when compared with 20gal/acre.

    Graphs and Data Tables:


    Plant Height (inches) across Time



    Total Nodes across Time


    Height to Node Ratio (inches) across Time


    Lbs of Lint/Acre Machine Harvest


    Lbs of Lint/Acre Hand Harvest


    Lbs of Lint/Acre on a Per Plant Basis


    Box Mapping:


    Lbs/Acre Across Positions On The Main Fruiting Axis


    Lbs lint cotton/AC of main axis fruiting sites compared to vegetative branches

    Pounds Lint Cotton/AC of Main Axis Fruiting Sites Compared To Vegetative Branches

    Lbs Lint Cotton/AC Across Zones

    Pounds Lint Cotton Per Acre Across Zones
    Accumulated Lbs. lint cotton/ac. across time and position

    Accumulated Pounds Lint Cotton Per Acre Across Time and Position
  • Field Tomatoes

    Increase your bottom line in field grown tomatoes with Ag1000<sup>®</sup>.
  • Jalapenos Followed by Wheat and Rye: Arizona Ag1000 Case Study

    Enterprises: Jalapenos, pumpkins, wheat, and rye

    Agriculture Issues: Very high salt, powdery mildew, and phytopthora

    Farm Information: Farm in Southeaster, Arizona. 17 acres. Subsurface Irrigation.

    Crop: Jalapeno "Dulce" (Capsicum annuum longum) transplants planted into winter rye grass cover crop

    Soil Type: Calcareous Silty Clay Loam with moderate salt levels
    Jalapenos on PlantJalapenos In FieldHarvested Jalapenos: Arizona


    Input

    Input Name

    Rate (g/ac)

    Cost/Unit

    Cost/Acre

    Microbes

    Ag1000™

    40

    $2.50/gal

    $100.00

    Fertilizers

    16-8-3-4 with Humate & Ca Lignonsulfate

    25

    $3.28/gal

    $65.60

    SuperCombo Foliar(w/Ag1000™)

    6

    $22.24

    $133.44

    Herbicides

    n/a

    n/a

    n/a

    n/a

    Pesticides

    n/a

    n/a

    n/a

    Other

    Total Costs

    $299.04


    Input

    Input Name

    Rate (g/ac)

    Cost/Unit

    Cost/Acre

    Microbes

    Ag1000™

    n/a

    n/a

    n/a

    Fertilizers

    16-8-3-4 with Humate & Ca Lignonsulfate

    50

    $3.28/gal

    $65.60

    SuperCombo Foliar

    6

    $22.24

    $133.44

    Herbicides

    n/a

    n/a

    n/a

    n/a

    Pesticides

    n/a

    n/a

    n/a

    Other

    Total Costs

    $199.04



    Year

    Field

    Crop

    Yield

    Tons/acre

    Ag1000™ Treatment/ac/yr

    2011

    Ag1000™

    Jalapenos

    20.5

    40

    2011

    No Ag1000™

    Jalapenos

    16.5

    None


    Worldwide, chile peppers (Capsicum annum L) represent a huge market for several crop-­‐producing areas in the desert Southwest (New Mexico, Arizona, Texas, and northern Mexico). In the United States, over 24,000 acres of chilies are produced and equate to over $120 million in production value (E. Babcock and Silvertooth 2010).

    In the desert southwest of Arizona, New Mexico, and California chili peppers are grown for both fresh vegetables and dried for a multitude of culinary products such as chili powder and paprika as well as seed for future production. As of the latest Agricultural census (NASS 2007), New Mexico is the leading state in chili production with 15,567 acres followed by Arizona with 5,510 acres.

    Ag1000™ is made up of three types of microorganisms; lactic acid bacteria, photosynthetic bacteria, and yeast. In Ag1000™ treated soils, polysaccharides and other beneficial organic acids and enzymes in the soil help build stable aggregate and soil structure. Soils are then able to absorb and retain moisture as well as cycle nutrients more productively. Nutrients are held in the upper layers of the soil and are held there by the soil cation exchange capacity (CEC).

    The CEC increases as aggregate formation matures because the aggregate are providing the surface area for the nutrients to adsorb. Ag1000™ efficiently breaks down organic matter into stable humus, a key component to aggregate formation, by reducing oxidative forces that primarily rob the soil of Carbon and Nitrogen in the form of methane and ammonia gases. Once you have healthier soils, healthier crops follow quickly. Microbes help in the breakdown of nutrients to plant usable forms,making plant uptake of nutrients quicker and more effective.

    This case study was done to test the application of Ag1000™ through the 2011-­‐ 2012 growing seasons; two fields were tested (one with Ag1000™, one without). The fields were planted with Jalapeno Chile Peppers in 2011, followed by Wheat and Rye. Soil nitrogen, phosphorus, potassium, zinc, boron, and organic matter were tested both pre-­‐planting and after harvest to compare concentrations. Five leaf analyses were also done throughout the season to test nutrient uptake by the plant. Irrigation and application of other inputs such as fertilizer, pesticides, herbicides, and compost were done based on the grower standards. Yield between the treated and non-­‐treated were also compared.

    Below the sodium (esp %) is compared for the full Ag1000™ trial, Years 2010 – 2012. Overall during the entire trial a decrease in sodium was observed in both fields, but more so in the Ag1000™ field, with the lowest being 4.4%. Organic matter is also compared showing the Ag1000™ being consistently higher than the control field and steadily increasing each year compared to no change in block 1.



    Organic Matter Comparison

    2011 Jalapeno Yield
    Soil Nutrient Comparison
    Soil Nutrient Comparison 2
    Sodium and Organic Matter Percentage
    2011 Leaf Sodium

    2011 Tissue NO3-N and PO4-P

    2011 Jalapeno Raw Data:

    Soil Sample Comparison: 3/25/11

    Field

    Treatment

    pH

    Ca

    Na

    K

    Mg

    Nitrate

    P

    OM

    ESP

    ppm

    ppm

    ppm

    ppm

    ppm

    ppm

    %

    %

    Block 1

    No Ag1000™

    8.6

    8800

    900

    680

    6.6

    8

    6.2

    3

    4.4

    Block 2-5

    Ag1000™

    8.3

    9000

    550

    980

    9.5

    18

    12

    2.9

    7.2


    Tissue Sample Comparison

    Date

    N

    P

    Total N

    K

    Ca

    Mg

    Total Mg

    Na

    ppm

    ppm

    %

    %

    %

    Ppm

    %

    %

    6/13/2011

    Block 1

    12900

    3000

    5.3

    4.7

    1.5

    94

    .42

    .08

    Block 2-5

    161000

    3800

    5.7

    4.9

    2.3

    130

    .69

    .03

    7/11/2011

    Block 1

    1600

    2300

    5.0

    4.0

    1.4

    110

    .41

    .01

    Block 2-5

    400

    2100

    4.1

    4.7

    1.1

    87

    .32

    .03

    7/25/2011

    Block 1

    8900

    1300

    5.5

    4.5

    1.80

    83

    .50

    .01

    Block 2-5

    11000

    1200

    5.5

    4.9

    1.70

    63

    .40

    .01

    8/8/2011

    Block 1

    2800

    900

    4.4

    4.2

    2.50

    92

    .55

    0.00

    Block 2-5

    2800

    1300

    4.7

    4.8

    1.80

    53

    .50

    0.01

    At Coyote Farms, the 2011 jalapeno Ag1000™ trial showed an increase in calcium, potassium, phosphorus, and magnesium as well as decreased pH and sodium in the soil samples. Leaf tissue samples showed an overall increase in all vital nutrients need for maximum yield, as well as a decrease in sodium concentration. It was also observed that jalapeno plants grew more bush-­‐like and grew multiple fruits per node, compared to one fruit per node. Little or no powdery mildew, phytopthora or bacterial leaf spot was observed in the Ag1000™ field, which are very common and hard to treat disease problems in peppers. In a study on the nitrogen fixing bacteria in Ag1000™ by A. Javaid, it was found that photosynthetic bacteria improved crop growth and yield by increasing photosynthesis, producing bioactive substances such as enzymes and hormones, and controlling soil diseases (A. Javaid 2009). Tissue nitrate was increased from the use of Ag1000™ from the enhanced decomposition of organic nitrogen by microorganisms into inorganic nitrogen that can be used by the plant.

    The main issue Coyote Farms was running into was high salts and white crusting on the surface of the soil. Because of the microbes’ ability to add organic matter and better structure the soil, less water is usually needed in Ag1000™ and fewer salts are added from irrigation water. Microorganisms like those in Ag1000™, also work against high salts in the soil by keeping salts from ionizing.

    Data Tables and Summaries for 2012 Wheat and Rye Trial:
    Wheat Soil Nutrient Comparisons 2012

    Wheat Soil Comparison

    Rye Soil Nutrient Comparison

    Rye Soil Nutrient Comparison 2

    2012 Wheat And Rye Raw Data:
    3/25/2012: Wheat

    Field

    Treatment

    pH

    Ca

    Na

    K

    Mg

    Nitrate

    P

    OM

    ESP

    ppm

    ppm

    ppm

    ppm

    ppm

    ppm

    %

    %

    Block 1

    No Ag1000™

    8.3

    8000

    870

    580

    460

    5.6

    7.2

    2.9

    7.7

    Block 2-5

    Ag1000™

    8.3

    7900

    510

    630

    470

    3.5

    8.5

    3

    4.7


    11/15/2012: Rye

    Field

    Treatment

    pH

    Ca

    Na

    K

    Mg

    Nitrate

    P

    OM

    ESP

    ppm

    ppm

    ppm

    ppm

    ppm

    ppm

    %

    %

    Block 1

    No Ag1000™

    8.6

    7700

    660

    670

    480

    13

    6.3

    2.9

    6.1

    Block 2-5

    Ag1000™

    8.3

    7900

    600

    1000

    470

    22

    14

    4

    5.4


    Following pumpkins in 2010 and jalapenos in 2011, the wheat and rye trials continued to show an overall decrease in sodium ESP (shown in first graph) in the Ag1000™ treated fields. Nutrients such as calcium, potassium, magnesium, nitrate, and phosphate were higher in Ag1000™ fields as well as a steady increase in organic matter throughout the three growing seasons. In 2010, when planted the pumpkins the Ag1000™ fields had an organic matter of 1%, by 2012 organic matter had increased to 4 when planted with rye grass (second graph)

    References:
    E. L. Babcock; J.C. Silvertooth. 2012. Soil Testing and Plant Analysis Relationships for Irrigated Chile Production. Communications in Soil Science and Plant Analysis. 2012;43(20):2651-­‐2668.

    NASS. 2007. USDA Census of Agriculture. http://www.agcensus.usda.gov/index.php




  • Melons

    Melons Grown With Ag1000™

    Ag1000™ is a ready-to-use soil conditioner comprised

    of Effective Microorganisms®. Ag1000™ allows for increased

    brix, a better nutrient exchange in your soil, and helps

    to improve drainage, aeration, moisture retention, and

    soil structure and decreases fertilizer requirements while

    maintaining or improving yield. Healthy soil leads to

    healthy plants – Ag1000™ provides your plant with essential

    vitamins, amino acids, and enzymes that improve plant

    vigor and enhance plant growth.


    Overall, Ag1000™ will increase your bottom line by

    increasing yields and production. Continued use of Ag1000™

    will improve nutrient availability, assist in balancing pH, and

    increase organic matter for improved moisture retention.


    TeraGanix® will provide training on the use of Ag1000™ and

    serve as a technical resource throughout the use of the

    product. We are proud to provide industry leading solutions

    for a multitude of needs and will serve as a partner in your

    business to assist in your growth by using our product

    knowledge and business philosophy.


    Suggested Application

    • Apply 3-5 gallons/acre/week in soil and 1.5-3 gallons/acre/week foliar

    Availability

    • Available in 55-gallon, 275-gallon, and in bulk delivery of over 1,000 gallons
    • Available in two forms: Conventional and Ag1000 Organic (OMRI Listed)
  • Onions

    Onions Grown With Ag1000™

    Ag1000™ provides onion growers with a solution to add to their nutrient programs that will help build soil and assist in nutrient efficiency. Below are three optional programs that are to be used in conjunction with an existing nutrient program. These programs may also be combined with some other soil building products such as Bokashi, compost, myccorhizae, and humates (liquid or granular).
    Starlight Onions New MexicoStarlight Onions New Mexico

    Anticipated benefits include:
    • Better drought tolerance
    • Increased nutrient efficiency
    • Lower salts in soils
    • Decreased fertilizer rates
    • Increased water retention in soil
    • Increased shelf life
    At seeding:
    Low: 5 gallons per acre
    Medium: 10 gallons per acre
    Intensive: 20 gallons per acre

    Onion sets: Dip transplants in a 3-5% Solution at time of transplanting.

    Drench: Alternately, you can drench or band with fertilizer mix at time of planting.

    Irrigated crop:
    Low: 0.5 gallons per acre per week foliar
    Medium: 1 gallon per acre per week foliar plus 1 gallon per acre in irrigation water.
    Intensive: Up to 5 gallons per acre per week foliar plus 1 gallon per acre in irrigation water
    .
    Grow-out:
    Low: 0.5 gallons per acre per week foliar
    Medium: 1 gallon per acre per week foliar plus 1 gallon per acre in irrigation water.
    Intensive: Up to 5 gallons per acre per week foliar plus 1 gallon per acre in irrigation water.


    For Ag1000™ Pricing and Availability, call toll free: 866.369.3678
  • Peanuts

    Growing peanuts? Soil tilth is crucial for high quality peanuts. Ag1000™ improves overall soil tilth and a whole lot more!
  • Peppers

    Grow great peppers with Ag1000™ Microbial Inoculant.
  • Pumpkins Case Study: Arizona

    Location:
    San Simon, AZ 85632-8049

    Contact Information:

    Brian Driscoll

    Farmed Acres: 17

    Enterprises
    Jalapenos, pumpkins, wheat, Rye

    Agriculture Issues: Very high salt and powdery mildew

    Farm Information:
    Crop: Pumpkin var “Spartan”

    Soil Type: Calcareous Silty Clay Loam with moderate salt levels
    Pumpkins In Field San Simon, AZPumpkin Plants San Simon, Arizona

    Input

    Input Name

    Rate (g/ac)

    Cost/Unit

    Cost/Acre

    Microbes

    Ag1000™

    40

    $2.50/gal

    $100.00

    Fertilizers

    16-8-3-4 with Humate & Ca Lignonsulfate

    20

    $3.28/gal

    $65.60

    SuperCombo Foliar(w/Ag1000™)

    n/a

    $22.24

    $133.44

    Herbicides

    n/a

    n/a

    n/a

    n/a

    Pesticides

    n/a

    n/a

    n/a

    Other

    Total Costs

    $299.04



    Input

    Input Name

    Rate (g/ac)

    Cost/Unit

    Cost/Acre

    Microbes

    Ag1000™

    n/a

    n/a

    n/a

    Fertilizers

    16-8-3-4 with Humate & Ca Lignonsulfate

    20

    $3.28/gal

    $65.60

    SuperCombo Foliar

    n/a

    n/a

    n/a

    Herbicides

    n/a

    n/a

    n/a

    n/a

    Pesticides

    n/a

    n/a

    n/a

    Other

    Total Costs

    $199.04


    Year

    Field

    Crop

    Yield

    40 ct. Boxes/acre

    Ag1000™ Treatment/ac/yr

    2010

    Ag1000™

    Pumpkins

    54.52

    40

    2010

    No Ag1000™

    Pumpkins

    45

    None


    This case study was done to test the application of Ag1000™. Two fields were tested, one with Ag1000™ and one without Ag1000™. Soil nitrogen, phosphorus, potassium, zinc, boron, and organic matter were tested both pre-planting and after harvest to compared concentrations. Five leaf analyses were also done throughout the season to test nutrient uptake by the plant. Irrigation and application of other inputs such as fertilizer, pesticides, herbicides, and compost were done based on the grower standards. Yield between the treated and non-treated were also compared.

    Date Tables and Summaries for 2010 and 2011:
    2010 Pumpkin Yields (Ag1000 vs Control)

    Soil Nutrient Comparison 1
    Soil Nutrient Comparison 2
    Sodium and Organic Matter Percentage

    2010 Raw Data:

    Soil Sample Comparison: 12/7/11

    Field

    Treatment

    pH

    Ca

    Na

    K

    Mg

    Nitrate

    P

    OM

    ESP

    ppm

    ppm

    ppm

    ppm

    ppm

    ppm

    %

    %

    Block 1

    No Ag1000™

    8.4

    5700

    1100

    810

    10

    14

    10

    .58

    12.1

    Block 2-5

    Ag1000™

    8.0

    5700

    600

    990

    14

    55

    18

    1.00

    7.1

    At Coyote Farms, the 2010 pumpkin Ag1000™ trial showed no increase in calcium, but showed significant increases in potassium, phosphorus, and magnesium as well as a decrease in pH and significant decrease in sodium in the soil samples. It was also observed that pumpkin plants were less likely to get deceased from powdery mildew and better moisture retention was observed in both the soil and the fruit itself. Fruit load was also greatly increased with the use of Ag1000™. In a study on the nitrogen fixing bacteria in Ag1000™ by A. Javaid, it was found that photosynthetic bacteria improved crop growth and yield by increasing photosynthesis, producing bioactive substances such as enzymes and hormones, and controlling soil decease (A. Javaid 2009).

    The main issue Coyote Farms was running into was high salts and white crusting on the surface of the soil. Because of the microbes’ ability to add organic matter and better structure the soil,less water is usually needed in Ag1000™ and fewer salts are added from irrigation water. Microorganisms like those in Ag1000™, also work against high salts in the soil by keeping salts from ionizing. Salt content was dramatically different between Block 1 (No Ag1000™) and Block 2-5 (Ag1000™) with Block 1 having an ESP of 12.1% versus 7.1% for Block 2-5.

  • Soybeans

    Ag1000® is a ready-to-use soil conditioner comprised of Effective Microorganisms. Ag1000® allows a better nutrient exchange in your soil and helps to improve drainage, aeration and soil structure and decrease fertilizer requirements while maintaining or improving yield. Healthy soil leads to healthy plants – Ag1000® provides your plant with essential vitamins, amino acids, and enzymes that improve plant vigor and enhance plant growth.

    Overall, Ag1000® will increase your bottom line by increasing yields and production. Continued use of Ag1000® will improve nutrient availability, assist in balancing pH, and increase organic matter for improved moisture retention.

    TeraGanix® will provide training on the use of Ag1000® and serve as a technical resource throughout the use of the product. We are proud to provide industry leading solutions for a multitude of needs and will serve as a partner in your business to assist in your growth by using our product knowledge and business philosophy.

    Application
    • Apply 5 gallons/acre at planting
    • Begin applying foliar sprays of 2 gallons/acre once you have a plant, continuing each week for 8 treatments
    • Apply 5 gallons/acre post-harvest
    APPLICATION TOTALS 26 GALLONS/ACRE/YEAR

    Availability
    • Available in 1 gallon, 5 gallon, 55 gallon, 275 gallon and in bulk delivery of over 1,000 gallons
    • Available in two forms: Conventional and Ag1000® Organic (OMRI Listed)
  • Sweet Potatoes

    Grow sweet potatoes with Ag1000®. Available in conventional formula or organic.